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lentiviral packaging vector pspax2, envelope vector pmd2g, and overexpression plasmid pcdh  (Thermo Fisher)


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    Thermo Fisher lentiviral packaging vector pspax2, envelope vector pmd2g, and overexpression plasmid pcdh
    Lentiviral Packaging Vector Pspax2, Envelope Vector Pmd2g, And Overexpression Plasmid Pcdh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/packaging+vectors+pmd2g/10__54097_slash_h62tgg30-35-34-40
    Average 90 stars, based on 1 article reviews
    lentiviral packaging vector pspax2, envelope vector pmd2g, and overexpression plasmid pcdh - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY.
    Article Snippet: Lentiviral vectors encoding the human ONECUT2, ACLY and FGF2 genes were constructed in pLV-puro or pLV-neo (Addgene). .. For the generation of targeted lentivirus, HEK-293T cells were transfected with the recombinant vectors and packaging vectors pMD2G and psPAX2 (Addgene plasmid #12259 and #12260) using Lipofectamine®3000 (Thermo Fisher Scientific). .. After 48 h, viral supernatant was harvested by centrifugation and filtered with a 0.45-μm filter (Millipore, MA, USA).

    Recombinant:

    Article Title: ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY.
    Article Snippet: Lentiviral vectors encoding the human ONECUT2, ACLY and FGF2 genes were constructed in pLV-puro or pLV-neo (Addgene). .. For the generation of targeted lentivirus, HEK-293T cells were transfected with the recombinant vectors and packaging vectors pMD2G and psPAX2 (Addgene plasmid #12259 and #12260) using Lipofectamine®3000 (Thermo Fisher Scientific). .. After 48 h, viral supernatant was harvested by centrifugation and filtered with a 0.45-μm filter (Millipore, MA, USA).

    Plasmid Preparation:

    Article Title: ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY.
    Article Snippet: Lentiviral vectors encoding the human ONECUT2, ACLY and FGF2 genes were constructed in pLV-puro or pLV-neo (Addgene). .. For the generation of targeted lentivirus, HEK-293T cells were transfected with the recombinant vectors and packaging vectors pMD2G and psPAX2 (Addgene plasmid #12259 and #12260) using Lipofectamine®3000 (Thermo Fisher Scientific). .. After 48 h, viral supernatant was harvested by centrifugation and filtered with a 0.45-μm filter (Millipore, MA, USA).



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